cd3 t cells Search Results


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Magcellect Human Cd3 T Cell Isolation Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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UMB-136 reverses HIV-1 latency from multiple cell models of HIV-1 latency. (A) Multiple J-Lat cell clones (6.3, 8.4, 9.2, and 10.4, <t>CD4+</t> T cell background) were treated with UMB-136 (2.5 or 5 μM) or JQ1 (1 μM). GFP-positive cells were acquired and analyzed by flow cytometry. Percentage of GFP-positive cells was determined. One representative of three independent experiments was shown. (B) THP89GFP cells (monocyte/macrophage background) were treated with UMB-136 (5 μM) or JQ1 (1 μM). GFP-positive cells were acquired and analyzed by flow cytometry. The HIV-reactivated cell population was indicated. (C) HIV-1 latency was established in the CD4+ T cells isolated from the peripheral blood mononuclear cells (PBMCs) of 2 donors, following a previously reported primary cell model (Vicente Planelles). Cells latently infected with wild-type HIV-1 NL4-3 viruses were treated with UMB-136 (2.5 μM) or JQ1 (1 μM). The viral mRNAs from newly produced HIV-1 viruses in supernatant were measured by qRT-PCR assay. (D) The same assay in (D) was conducted using the CD4+ T cells isolated from the tonsillar mononuclear cells (TMCs) of 2 donors. Results were presented as mean ± s.e.m. ( n = 3 ); * p < 0.05, t -test.
Primary Cd4 Helper T Cells, supplied by Sanguine Biosciences Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc cd3+ t-cells
UMB-136 reverses HIV-1 latency from multiple cell models of HIV-1 latency. (A) Multiple J-Lat cell clones (6.3, 8.4, 9.2, and 10.4, <t>CD4+</t> T cell background) were treated with UMB-136 (2.5 or 5 μM) or JQ1 (1 μM). GFP-positive cells were acquired and analyzed by flow cytometry. Percentage of GFP-positive cells was determined. One representative of three independent experiments was shown. (B) THP89GFP cells (monocyte/macrophage background) were treated with UMB-136 (5 μM) or JQ1 (1 μM). GFP-positive cells were acquired and analyzed by flow cytometry. The HIV-reactivated cell population was indicated. (C) HIV-1 latency was established in the CD4+ T cells isolated from the peripheral blood mononuclear cells (PBMCs) of 2 donors, following a previously reported primary cell model (Vicente Planelles). Cells latently infected with wild-type HIV-1 NL4-3 viruses were treated with UMB-136 (2.5 μM) or JQ1 (1 μM). The viral mRNAs from newly produced HIV-1 viruses in supernatant were measured by qRT-PCR assay. (D) The same assay in (D) was conducted using the CD4+ T cells isolated from the tonsillar mononuclear cells (TMCs) of 2 donors. Results were presented as mean ± s.e.m. ( n = 3 ); * p < 0.05, t -test.
Cd3+ T Cells, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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UMB-136 reverses HIV-1 latency from multiple cell models of HIV-1 latency. (A) Multiple J-Lat cell clones (6.3, 8.4, 9.2, and 10.4, <t>CD4+</t> T cell background) were treated with UMB-136 (2.5 or 5 μM) or JQ1 (1 μM). GFP-positive cells were acquired and analyzed by flow cytometry. Percentage of GFP-positive cells was determined. One representative of three independent experiments was shown. (B) THP89GFP cells (monocyte/macrophage background) were treated with UMB-136 (5 μM) or JQ1 (1 μM). GFP-positive cells were acquired and analyzed by flow cytometry. The HIV-reactivated cell population was indicated. (C) HIV-1 latency was established in the CD4+ T cells isolated from the peripheral blood mononuclear cells (PBMCs) of 2 donors, following a previously reported primary cell model (Vicente Planelles). Cells latently infected with wild-type HIV-1 NL4-3 viruses were treated with UMB-136 (2.5 μM) or JQ1 (1 μM). The viral mRNAs from newly produced HIV-1 viruses in supernatant were measured by qRT-PCR assay. (D) The same assay in (D) was conducted using the CD4+ T cells isolated from the tonsillar mononuclear cells (TMCs) of 2 donors. Results were presented as mean ± s.e.m. ( n = 3 ); * p < 0.05, t -test.
Magnetically Depleted Of Cd8− Cells, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc stemsep human cd3+ t cell depletion
UMB-136 reverses HIV-1 latency from multiple cell models of HIV-1 latency. (A) Multiple J-Lat cell clones (6.3, 8.4, 9.2, and 10.4, <t>CD4+</t> T cell background) were treated with UMB-136 (2.5 or 5 μM) or JQ1 (1 μM). GFP-positive cells were acquired and analyzed by flow cytometry. Percentage of GFP-positive cells was determined. One representative of three independent experiments was shown. (B) THP89GFP cells (monocyte/macrophage background) were treated with UMB-136 (5 μM) or JQ1 (1 μM). GFP-positive cells were acquired and analyzed by flow cytometry. The HIV-reactivated cell population was indicated. (C) HIV-1 latency was established in the CD4+ T cells isolated from the peripheral blood mononuclear cells (PBMCs) of 2 donors, following a previously reported primary cell model (Vicente Planelles). Cells latently infected with wild-type HIV-1 NL4-3 viruses were treated with UMB-136 (2.5 μM) or JQ1 (1 μM). The viral mRNAs from newly produced HIV-1 viruses in supernatant were measured by qRT-PCR assay. (D) The same assay in (D) was conducted using the CD4+ T cells isolated from the tonsillar mononuclear cells (TMCs) of 2 donors. Results were presented as mean ± s.e.m. ( n = 3 ); * p < 0.05, t -test.
Stemsep Human Cd3+ T Cell Depletion, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biological Specialty Corporation cd4 t cells
UMB-136 reverses HIV-1 latency from multiple cell models of HIV-1 latency. (A) Multiple J-Lat cell clones (6.3, 8.4, 9.2, and 10.4, <t>CD4+</t> T cell background) were treated with UMB-136 (2.5 or 5 μM) or JQ1 (1 μM). GFP-positive cells were acquired and analyzed by flow cytometry. Percentage of GFP-positive cells was determined. One representative of three independent experiments was shown. (B) THP89GFP cells (monocyte/macrophage background) were treated with UMB-136 (5 μM) or JQ1 (1 μM). GFP-positive cells were acquired and analyzed by flow cytometry. The HIV-reactivated cell population was indicated. (C) HIV-1 latency was established in the CD4+ T cells isolated from the peripheral blood mononuclear cells (PBMCs) of 2 donors, following a previously reported primary cell model (Vicente Planelles). Cells latently infected with wild-type HIV-1 NL4-3 viruses were treated with UMB-136 (2.5 μM) or JQ1 (1 μM). The viral mRNAs from newly produced HIV-1 viruses in supernatant were measured by qRT-PCR assay. (D) The same assay in (D) was conducted using the CD4+ T cells isolated from the tonsillar mononuclear cells (TMCs) of 2 donors. Results were presented as mean ± s.e.m. ( n = 3 ); * p < 0.05, t -test.
Cd4 T Cells, supplied by Biological Specialty Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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NeoTX Inc cd3+ t cells
UMB-136 reverses HIV-1 latency from multiple cell models of HIV-1 latency. (A) Multiple J-Lat cell clones (6.3, 8.4, 9.2, and 10.4, <t>CD4+</t> T cell background) were treated with UMB-136 (2.5 or 5 μM) or JQ1 (1 μM). GFP-positive cells were acquired and analyzed by flow cytometry. Percentage of GFP-positive cells was determined. One representative of three independent experiments was shown. (B) THP89GFP cells (monocyte/macrophage background) were treated with UMB-136 (5 μM) or JQ1 (1 μM). GFP-positive cells were acquired and analyzed by flow cytometry. The HIV-reactivated cell population was indicated. (C) HIV-1 latency was established in the CD4+ T cells isolated from the peripheral blood mononuclear cells (PBMCs) of 2 donors, following a previously reported primary cell model (Vicente Planelles). Cells latently infected with wild-type HIV-1 NL4-3 viruses were treated with UMB-136 (2.5 μM) or JQ1 (1 μM). The viral mRNAs from newly produced HIV-1 viruses in supernatant were measured by qRT-PCR assay. (D) The same assay in (D) was conducted using the CD4+ T cells isolated from the tonsillar mononuclear cells (TMCs) of 2 donors. Results were presented as mean ± s.e.m. ( n = 3 ); * p < 0.05, t -test.
Cd3+ T Cells, supplied by NeoTX Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Astarte Biologics cd3+ t cells
UMB-136 reverses HIV-1 latency from multiple cell models of HIV-1 latency. (A) Multiple J-Lat cell clones (6.3, 8.4, 9.2, and 10.4, <t>CD4+</t> T cell background) were treated with UMB-136 (2.5 or 5 μM) or JQ1 (1 μM). GFP-positive cells were acquired and analyzed by flow cytometry. Percentage of GFP-positive cells was determined. One representative of three independent experiments was shown. (B) THP89GFP cells (monocyte/macrophage background) were treated with UMB-136 (5 μM) or JQ1 (1 μM). GFP-positive cells were acquired and analyzed by flow cytometry. The HIV-reactivated cell population was indicated. (C) HIV-1 latency was established in the CD4+ T cells isolated from the peripheral blood mononuclear cells (PBMCs) of 2 donors, following a previously reported primary cell model (Vicente Planelles). Cells latently infected with wild-type HIV-1 NL4-3 viruses were treated with UMB-136 (2.5 μM) or JQ1 (1 μM). The viral mRNAs from newly produced HIV-1 viruses in supernatant were measured by qRT-PCR assay. (D) The same assay in (D) was conducted using the CD4+ T cells isolated from the tonsillar mononuclear cells (TMCs) of 2 donors. Results were presented as mean ± s.e.m. ( n = 3 ); * p < 0.05, t -test.
Cd3+ T Cells, supplied by Astarte Biologics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


UMB-136 reverses HIV-1 latency from multiple cell models of HIV-1 latency. (A) Multiple J-Lat cell clones (6.3, 8.4, 9.2, and 10.4, CD4+ T cell background) were treated with UMB-136 (2.5 or 5 μM) or JQ1 (1 μM). GFP-positive cells were acquired and analyzed by flow cytometry. Percentage of GFP-positive cells was determined. One representative of three independent experiments was shown. (B) THP89GFP cells (monocyte/macrophage background) were treated with UMB-136 (5 μM) or JQ1 (1 μM). GFP-positive cells were acquired and analyzed by flow cytometry. The HIV-reactivated cell population was indicated. (C) HIV-1 latency was established in the CD4+ T cells isolated from the peripheral blood mononuclear cells (PBMCs) of 2 donors, following a previously reported primary cell model (Vicente Planelles). Cells latently infected with wild-type HIV-1 NL4-3 viruses were treated with UMB-136 (2.5 μM) or JQ1 (1 μM). The viral mRNAs from newly produced HIV-1 viruses in supernatant were measured by qRT-PCR assay. (D) The same assay in (D) was conducted using the CD4+ T cells isolated from the tonsillar mononuclear cells (TMCs) of 2 donors. Results were presented as mean ± s.e.m. ( n = 3 ); * p < 0.05, t -test.

Journal: Frontiers in Microbiology

Article Title: A Novel Bromodomain Inhibitor Reverses HIV-1 Latency through Specific Binding with BRD4 to Promote Tat and P-TEFb Association

doi: 10.3389/fmicb.2017.01035

Figure Lengend Snippet: UMB-136 reverses HIV-1 latency from multiple cell models of HIV-1 latency. (A) Multiple J-Lat cell clones (6.3, 8.4, 9.2, and 10.4, CD4+ T cell background) were treated with UMB-136 (2.5 or 5 μM) or JQ1 (1 μM). GFP-positive cells were acquired and analyzed by flow cytometry. Percentage of GFP-positive cells was determined. One representative of three independent experiments was shown. (B) THP89GFP cells (monocyte/macrophage background) were treated with UMB-136 (5 μM) or JQ1 (1 μM). GFP-positive cells were acquired and analyzed by flow cytometry. The HIV-reactivated cell population was indicated. (C) HIV-1 latency was established in the CD4+ T cells isolated from the peripheral blood mononuclear cells (PBMCs) of 2 donors, following a previously reported primary cell model (Vicente Planelles). Cells latently infected with wild-type HIV-1 NL4-3 viruses were treated with UMB-136 (2.5 μM) or JQ1 (1 μM). The viral mRNAs from newly produced HIV-1 viruses in supernatant were measured by qRT-PCR assay. (D) The same assay in (D) was conducted using the CD4+ T cells isolated from the tonsillar mononuclear cells (TMCs) of 2 donors. Results were presented as mean ± s.e.m. ( n = 3 ); * p < 0.05, t -test.

Article Snippet: THP89GFP and Primary CD4 + helper T cells (Sanguine Biosciences and Lonza) were cultured and maintained, respectively, in complete medium (RPMI 1640 with 10% FBS, 1x glutamine, 1x MEM non-essential amino acid solution, and 20 mM HEPES).

Techniques: Clone Assay, Flow Cytometry, Isolation, Infection, Produced, Quantitative RT-PCR